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γ-Glutamyltransferase (GGT) Test Kit (Velocity method)

The γ-Glutamyltransferase (GGT) test kit is intended for the accurate and direct quantitative measurement of GGT activity in human plasma. GGT measurement is useful in the diagnosis of hepatobiliary disease.
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Test principle

In this test kit, soluble L-γ-glutamyl-3-carboxyl-p-nitroaniline (Glupac) serves as a substrate, and glycyglycine is designed to be a receptor of γ-glutamyl. γ-glutamyl can be transferred to glycyglycine molecules when catalyzed by GGT, producing yellow-colored 3-hydroxyl-p-nitroaniline. The reaction can induce an increase of absorbance at 405 nm, and the increasing rate is proportional to the activity of GGT.

 

Product Features

1.    Linear range of GGT activity: [2, 500] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.90

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.05  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.05 while testing the specific GGT sample of activity of 100 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 6.0%; inter CV% ≤8.0%.     


Technical Parameters

Temperature

37

Optical path length (OPL)

1.0 cm

Main wavelength

405 nm

Secondary wavelength

480 nm (Optional)

Method

Velocity method

Reaction direction

Positive

Sample

10 μL

R1

200 μL

R2

50 μL

Delay time

100 s

Reaction time

180 s

Reference range of GGT activity: 5 – 50 U/L.

 

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml


Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.



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