Test principle

In alkaline solution, peptide bonds in protein combine with Cu²⁺, generating blue violet compounds. The absorbance of these compounds at 550 nm is proportional to the quantity of peptide bonds, and thus concentration of total protein can be calculated from the measured absorbance.

Product Features

1.    Linear range of TP level: [5, 90] g/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.30  

3.    Sensitivity

The change in absorbance (ΔmA) ≥ 0.50 while testing the specific TP sample of concentration of 48.9 g/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of TP level: 60 – 83 g/L.

Packing Specifications

R1: 1×40 ml, R2: 2×20 ml

R1: 4×40 ml, R2: 8×20 ml

R1: 1×100 ml, R2: 2×50 ml

R1: 2×100 ml, R2: 4×50 ml

R1: 3×100 ml, R2: 6×50 ml

Storage and stability

Unopened: Store test kits at 10 - 30℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 10 - 30℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.