Test principle

Urea is first oxidized in the presence of uricase, generating allantoin and H₂O₂. H₂O₂ reacts further with 4-AAP and DHBS to produce a red colored product, the concentration of which is proportional to the UA level.

The test principle is shown as followings:

The increasing rate of absorbance of red quinochrome at the wavelength of 520 nm is proportional to the concentration of UA.  

Performance characteristics

1.    Linear range of UA level: [4, 1000] μmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.40  

3.    Sensitivity

The change in absorbance (ΔmA) ≥ 0.10 while testing the specific UA sample of concentration of 343 μmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of UA level: 58.7 – 415.5 μmol/L.

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.