Test principle

a-Hydroxybutyrate dehydrogenase (a-HBD) catalyzes the reversible reaction:

a-Ketobutyrate+NADH+ H+   α-HBD   α-Hydroxybutyrate+NAD+

The reaction is monitored spectrophotometrically by measuring the decrease in NADH at 340 nm, and the decreasing rate of absorbance is proportional to a-HBD activity.

Product Features

1.    Linear range of a-HBD activity: [3, 800] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≥ 0.90

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.02  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.03 while testing the specific a-HBD sample of activity of 274 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of a-HBD activity: 81 – 219 U/L.

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.