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Inorganic Phosphorus (PHOS) Test Kit (UV spectrophotometric method)

The inorganic phosphorus (PHOS) test kit is intended for the accurate and direct quantitative measurement of inorganic phosphorus in human plasma. This test is mainly used in the diagnosis of renal dysfunction, bone disease and parathyroid disease.
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Test principle

In an acidic environment, inorganic phosphorus reacts with ammonium molybdate, generating ammonium phosphomolybdate complex which shows absorption at the wavelength of 340 nm. The absorbance is proportional to the concentration of inorganic phosphorus.


Product Features

1.    Linear range of P level: [0.30, 4.00] mmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 1.00  

3.    Sensitivity

The change in absorbance (ΔA) ≥ 0.01 while testing the specific P sample of concentration of 1.71 mmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

  

Technical Parameters

Temperature

37

Optical path length (OPL)

1.0 cm

Main wavelength

340 nm

Secondary wavelength

700 nm (Optional)

Method

End-point method

(single reagent)

Reaction direction

Positive

Sample

5 μL

Reagent

300 μL

Reaction time

300 s

Reference range of P level: 0.30 – 4.00 mmol/L.


Packing Specifications

R1: 1×40 ml, R2: 2×20 ml

R1: 4×40 ml, R2: 8×20 ml

R1: 1×100 ml, R2: 2×50 ml

R1: 2×100 ml, R2: 4×50 ml

R1: 3×100 ml, R2: 6×50 ml


Storage and stability

Unopened: Store test kits at 10 - 30℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 10 - 30℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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