Test principle

In this kit, a-AMY activity is determined using a coupled enzymatic assay, which results in a colorimetric (405 nm) product, proportional to the amount of substrate, ethylidene-pNP-G7 (EPS), cleaved by the a-AMY. The reactions are shown as followings:

G=glucose, pNP=p-nitrophenol

Product Features

1.    Linear range of a-AMY activity: [5, 1800] U/L

Linear correlation coefficient ∣r∣≥ 0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.30

3.    Changing rate of absorbance of blank ΔA/min ≤ 0.02  

4.    Sensitivity

Changing rate of absorbance of blank ΔmA/min ≥ 0.03 while testing the specific a-AMY sample of activity of 177 U/L.

5.    Accuracy

Relative deviation is within ±10%.

6.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

Technical Parameters

Reference range of a-AMY activity: 12 – 223 U/L.

Packing Specifications

R1: 1×40 ml, R2: 1×10 ml

R1: 2×40 ml, R2: 1×20 ml

R1: 2×60 ml, R2: 2×15 ml

R1: 2×80 ml, R2: 2×20 ml

R1: 4×60 ml, R2: 4×15 ml

R1: 3×80 ml, R2: 3×20 ml

Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.