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Carbon Dioxide (CO2) Test Kit (Colorimetric method)

The carbon dioxide (CO2) test kit is intended for the accurate and direct quantitative measurement of CO2 concentration in human plasma. CO2 test is used in the diagnosis of acid-base balance. Unusual increase or decrease indicates the acid-base disorders of respiratory system or metabolic system.
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Test principle

According to Henderson-Hasselbalch equation (pH = pK + log[HCO3-]/[pCO2]), it can be considered that pH mainly depends on concentration of HCO3- when PCO2 is less than 0.5Kpa. HCO3- reacts with phenol red, showing a yellow color and an absorption at 546 nm. The absorbance is linear with the concentration of HCO3-, which can be dtermined by comparing with the reference solution.      

    

Product Features

1.    Linear range of CO2 level: [0, 50] mmol/L

Linear correlation coefficient ∣r∣≥0.990

Linear deviation ≤ ± 10.00%

2.    Absorbance of blank ≤ 0.30  

3.    Sensitivity

The change in absorbance (ΔmA) ≥ 2.00 while testing the specific sample of concentration of 25.0 mmol/L.

4.    Accuracy

Relative deviation is within ±10%.

5.    Precision

Intra CV% ≤ 5.0%; inter CV% ≤ 5.0%.

 

Technical Parameters

Temperature

37

Optical path length (OPL)

1.0 cm

Main wavelength

546 nm

Secondary wavelength

-

Method

End-point method

(single reagent)

Reaction direction

Positive

Sample

15 μL

R1

150 μL

R2

150 μL

Reaction time

600 s

Reference range of CO2 level: 23 – 29 mmol/L.

 

Packing Specifications

R1: 1×20 ml, R2: 1×20 ml

R1: 1×40 ml, R2: 1×40 ml

R1: 1×60 ml, R2: 1×60 ml

R1: 1×80 ml, R2: 1×80 ml

R1: 2×40 ml, R2: 2×40 ml

R1: 2×80 ml, R2: 2×80 ml

R1: 4×40 ml, R2: 4×40 ml


Storage and stability

Unopened: Store test kits at 2 - 8℃ protected from light. Stable to expiration date printed on individual vials (for 15 months).

Opened: Store test kits at 2 - 8℃ protected from light. Stable for 1 month if kept capped from original container and free from contamination.

Do not freeze the test kit.

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