Measurement of Type B natriuretic peptide in heparin and K2 EDTA plasmaClinical Biochemistry，2010:in press
Background
Ethylene diamine tetraacetic acid (EDTA) plasma is the only suitable specimen recommended by the manufacturers to be used in the determination of BNP. It appears crucial to evaluate if more conventional heparin plasma samples could be reliably used for BNP determination. Aim of this study was to evaluate the use of heparin plasma sample for BNP determination.
Methods
Venous blood from 42 consecutive patients admitted at the division of cardiology was collected in two test tubes, with K2-EDTA (Group 1) and lithium heparin with gel separator (Group 2) and analysed within 20 min of blood collection.
Results
Statistical analysis showed a significant difference between Group 1 and Group 2 (p < 0.0001). Sample collected in K2-EDTA showed a significant underestimation when compared to lithium–heparin.
Conclusions
Our data showed that BNP could not be dosed on different collection tubes without altering the results. In our experimental conditions, interestingly we found that BNP levels are significantly lower if measured in EDTA plasma.
译文：
在肝素抗凝和EDTA-K2抗凝血浆中测定B型利钠肽（BNP）。
背景：在测定BNP中，生产厂家往往推荐EDTA抗凝血浆最为唯一适合的测定标本。更加便捷分离血浆和血细胞的肝素抗凝血是否可以用于测定BNP是一个值得研究的问题。我们研究的目的就是评价使用肝素抗凝血浆是否可以测定BNP的含量。
方法：收集了心脏病室的42名被连续观察的病人的血浆，分别采用EDTA-K2抗凝（第一组）和带有分离胶的肝素抗凝血浆（第二组）。在血浆收集的20分钟内测定。
结果：统计结果显示两组之间存在明显差异（P<0.0001）。EDTA-K2抗凝标本的结果明显低于肝素抗凝的结果。
结论：数据表明利钠肽B在不同的抗凝管收集后，测定结果会出现差异。在我们的试验结果里，如果采用EDTA-K2血浆的话，BNP的测定结果明显偏低。
摘自《DXY》 |
